Fig. 5.

Flow cytometry charts of staining of smooth muscle α-actin (A) and smooth muscle myosin heavy chain (B). Purple peaks in A and B represent nonstained control. Pink peak in A represents positive stain of smooth muscle α-actin. Red peak in B represents positive stain of smooth muscle myosin heavy chain. M1, cutoff point for estimating percentage of positive stain. Purity was 97.41% for smooth muscle α-actin-stained cells (A) and 91.23% for smooth muscle myosin heavy chain-stained cells (B).