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. Author manuscript; available in PMC: 2013 Dec 30.
Published in final edited form as: Am J Physiol Heart Circ Physiol. 2005 Nov 18;290(4):10.1152/ajpheart.00826.2005. doi: 10.1152/ajpheart.00826.2005

Fig. 6.

Fig. 6

A: typical smoothelin Western blot from porcine coronary smooth muscle cells (a), A1WT coronary smooth muscle cells at passage 2 (b), A1KO coronary smooth muscle cells at passage 2 (c), A1WT aorta tissue (d), and porcine coronary artery endothelial cells (e). B: smoothelin Western blot from different passages of A1WT and A1KO coronary smooth muscle cells (CSMC): porcine coronary artery smooth muscle cells used as positive control (1), A1KO coronary endothelial cells used as negative control (2), A1WT CSMC at passage 3 (3), A1KO CSMC at passage 3 (4), A1WT CSMC at passage 7 (5), A1KO CSMC at passage 7 (6), A1WT CSMC at passage 11 (7), and A1KO CSMC at passage 11 (8). C: Western blot samples in B in the presence of smoothelin blocking peptide. D: Western immunoblotting of A1AR from A1WT and A1KO brain and CSMC samples. A ~36-kDa protein band was detected in A1WT brain and CSMC samples, but not in A1KO brain. In the presence of A1AR-blocking peptide, bands of protein from brain and CSMC of A1WT were completely abolished. Results are shown from 1 experiment that is representative of 3 independent experiments.