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. Author manuscript; available in PMC: 2013 Dec 30.
Published in final edited form as: J Biol Chem. 2006 Jun 27;281(34):10.1074/jbc.M603877200. doi: 10.1074/jbc.M603877200

FIGURE 5. Hic-5/SERT interactions are regulated by 5HT.

FIGURE 5

A, immunoprecipitation of SERT from mouse platelets. Mouse platelets were incubated with either 10 mm 5HT, 1 μm citalopram, 1 μm citalopram + 10 μm 5HT, or PBS (vehicle control) for 30 min at 37 °C, and SERT was immunoprecipitated as described under “Experimental Procedures.” Averaged data from four independent experiments are presented ± S.E. (*, p < 0.05 and **, p < 0.01, paired Student’s t test). B, translocation of Hic-5 between the different cytoskeletal compartments in the presence of 5HT. Mouse platelets were incubated with 10 μm 5HT, lysed with 1% Triton X-100, and the CS and MS pools were purified by differential fractionation. Averaged data of three independent experiments are presented.