TABLE 1.
Accuracy of the automated sample preparation protocol by use of HIV-1 controls and their dilutions
| Vial no. | HIV RNA (no. of copies/ml)
|
Log expected results/actual results | |
|---|---|---|---|
| Expected resultsa | Actual results | ||
| 1 | 1.0 × 102 | 1.9 × 102 | −0.28 |
| 2 | 5.0 × 102 | 2.5 × 102 | 0.30 |
| 3 | 1.0 × 103 | 7.6 × 102 | 0.12 |
| 4 | 2.5 × 104 | 1.2 × 104 | 0.32 |
| 5 | 5.0 × 104 | 3.3 × 104 | 0.19 |
a
The values for viral stock material were determined by the Roche COBAS AMPLICOR HIV-1 MONITOR (version 1.5) assay, and viral loads were adjusted to the specified concentrations by dilution in human plasma.