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. 2004 Apr;42(4):1439–1443. doi: 10.1128/JCM.42.4.1439-1443.2004

TABLE 2.

Interassay variation of results obtained by the automated versus the manual sample extraction protocol

Sample no. Mean no. of HIV-1 RNA copies/ml detected by the following protocol:
SD
Coefficient of variation (%)
Automateda Manualb Automated Manual Automated Manual
1 8.7 × 101 1.1 × 102 5.8 × 101 8.0 × 101 66 69
2 4.3 × 103 6.2 × 103 2.3 × 103 2.2 × 103 53 36
3 8.0 × 103 1.3 × 104 2.9 × 103 5.9 × 103 36 46
4 9.8 × 103 5.7 × 103 2.8 × 103 2.4 × 103 28 41
5 1.9 × 104 1.5 × 104 6.2 × 103 3.7 × 103 32 24
6 2.0 × 104 2.0 × 104 8.9 × 103 1.9 × 103 44 10
7 2.8 × 104 2.9 × 104 8.1 × 103 7.9 × 103 29 27
8 6.6 × 104 5.3 × 104 1.4 × 103 2.6 × 104 21 48
a

Automated sample preparation followed by the LCx HIV RNA Quantitative assay.

b

Manual sample preparation followed by the LCx HIV RNA Quantitative assay.