TABLE 3.
Intra-assay variation of results obtained by the automated versus the manual sample preparation protocola
| Sample no. | Mean no. of HIV-1 RNA copies/ml detected by the following protocol:
|
SD
|
Coefficient of variation (%)
|
|||
|---|---|---|---|---|---|---|
| Automatedb | Manualc | Automated | Manual | Automated | Manual | |
| 1 | 9.7 × 102 | 9.3 × 102 | 2.1 × 102 | 1.7 × 102 | 22 | 18 |
| 2 | 7.3 × 103 | 5.1 × 103 | 1.8 × 103 | 3.6 × 102 | 25 | 7 |
| 3 | 3.5 × 104 | 1.6 × 104 | 8.4 × 103 | 2.6 × 103 | 24 | 17 |
| 4 | 5.1 × 104 | 3.6 × 104 | 3.6 × 103 | 6.8 × 103 | 7 | 19 |
a
Data are from five replicates of one run.
b
Automated sample preparation followed by the LCx HIV RNA Quantitative assay.
c
Manual sample preparation followed by the LCx HIV RNA Quantitative assay.