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. Author manuscript; available in PMC: 2014 Oct 30.
Published in final edited form as: J Neurosci Methods. 2013 Sep 8;220(1):10.1016/j.jneumeth.2013.08.016. doi: 10.1016/j.jneumeth.2013.08.016

Figure 4. Double-labeling by FLICA and retrograde labeling tracer DTMR in whole-mounted larval lamprey brain at 2 weeks after spinal cord transection.

Figure 4

A, Retrograde labeling tracer DTMR (red) shows neurons in larval lamprey brain 2 weeks after application at the time of transection. B, Activated caspases in identified reticulospinal neurons, such as M1 and I1 neurons, revealed by FAM-VAD-FMK labeling (green, FLICA labeling) in the same brain used in A. Scale bar: 200 μm.