Skip to main content
. Author manuscript; available in PMC: 2014 Oct 30.
Published in final edited form as: J Neurosci Methods. 2013 Sep 8;220(1):10.1016/j.jneumeth.2013.08.016. doi: 10.1016/j.jneumeth.2013.08.016

Figure 6. Caspase inhibitor Z-VAD-FMK can inhibit FLICA labeling in lamprey brains.

Figure 6

A, FLICA labeling in the whole-mounted brain of a larval lamprey at 2 weeks post-transection. B, The fluorescent intensity of FLICA labeling was greatly reduced by pre-incubation with the unlabeled caspase inhibitor Z-VAD-FMK in the larval lamprey at 2 weeks post-transection. C and D, Higher maginification images from A and B. E, Effect of pre-incubation with unlabeled caspase inhibitor Z-VAD-FMK on intensity of FLICA labeling (means + SD). Pre-incubation reduced the intensity of FLICA labeling approximately 50% (p<0.0001, n=11 for FLICA labeling, n=20 for Z-VAD-FMK with FLICA labeling). Scale bar: 100 μm.