TABLE 1.
Comparison of two PCR methods (using artificial samples) in the presence of human DNA
| Primer set | No. of positive reactions/no. of reactions performed with DNA of:
|
|||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| AF
|
Blood or placenta
|
|||||||||
| 2a | 1 | 0.5 | 0.1 | 0.05 | 2 | 1 | 0.5 | 0.1 | 0.05 | |
| B22-B23 | 8/8 | 8/8 | 8/8 | 6/8 | 3/8b | 8/8 | 8/8 | 8/8b | 6/16b | 2/16b |
| T1-T4 | 8/8 | 8/8 | 7/8 | 4/8 | 2/8b | 8/8 | 6/8b | 4/8b | 2/16b | 0/16 |
a
Number of PGEs per PCR tube. Theoretically, these values would correspond to 10, 5, 2.5, 0.5, and 0.25 PGE/ml of AF, to 14, 7, 3.5, 0.7, and 0.35 PGE/ml of blood, and to ∼60, 30, 15, 3, and 1.5 PGE/g of placenta (from left to right, respectively).
b
Bands of low staining intensity in ethidium bromide-stained gels.