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. Author manuscript; available in PMC: 2014 Nov 26.
Published in final edited form as: Biochemistry. 2013 Nov 11;52(47):10.1021/bi401275v. doi: 10.1021/bi401275v

Figure 2.

Figure 2

Autoradiogram of a 15% SDS-polyacrylamide gel (100 V, 2 h) illustrating the incorporation of biphenyl-phenylalanine derivatives into positions 16 (upper panel) and 49 (lower panel) of DHFR. Lane 1, wild-type DHFR expression; lane 2, modified DHFR DNA in the presence of abbreviated suppressor tRNACUA-COH; lane 3, incorporation of amino acid A; lane 4, incorporation of amino acid B; lane 5, incorporation of amino acid C; lane 6, incorporation of amino acid D. Phosphorimager analysis was performed using an Amersham Biosciences Storm 820 equipped with ImageQuant version 5.2 software from Molecular Dynamics.