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. Author manuscript; available in PMC: 2014 Nov 26.
Published in final edited form as: Biochemistry. 2013 Nov 11;52(47):10.1021/bi401275v. doi: 10.1021/bi401275v

Table 1.

MALDI-MS Analysis of Tryptic Digests of Wild-type and Modified DHFRs (Biphenyl-phenylalanine C in Position 49). Position 49 is Denoted in graphic file with name nihms539693ig1.jpg

position peptide sequence MALDI-MS analysis,
molecular mass, Da
wild-type modified
Est MS Est MS
13– 32 VIGMENAMPWNLPADLAWFK 2304 2304.1 2304 2304.3
34–44 NTLNKPVIMGR 1242 1242.8 1242 1242.9
45–57 graphic file with name nihms539693t1.jpg 1506 1505.5 1718 1718.0
59–71 NIILSSQPGTDDR 1415 1415.5 1415 1415.9
72–76 VTWVK 632 632.5 632 634.3
77–98 SVDEAIAAC#GDVPEIMVIGGGR 2216 2216.4 2216 2217.1
99–106 VYEQFLPK 1023 1023.3 1023 1023.7
#

cysteine was alkylated with 2-iodoacetamide.