. Author manuscript; available in PMC: 2014 Nov 26.

Published in final edited form as: Biochemistry. 2013 Nov 11;52(47):10.1021/bi401275v. doi: 10.1021/bi401275v

Table 2.

Enzymatic Activities of Modified DHFRs

DHFR	position 16 (Met) (%)	position 49 (Ser) (%)	position 115 (Ile) (%)

wild type DHFR	100	100	100
A	170 ± 12^a	67 ± 5	91 ± 3
B	197 ± 12	68 ± 5	25 ± 2
C	171 ± 9	77 ± 6	82 ± 3
D	192 ± 11	76 ± 6	12 ± 2

Standard deviation based on data from three experiments.