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. Author manuscript; available in PMC: 2015 Jan 1.
Published in final edited form as: Arch Biochem Biophys. 2013 Nov 4;541:10.1016/j.abb.2013.10.021. doi: 10.1016/j.abb.2013.10.021

Figure 2.

Figure 2

Figure 2

AhR:ARNT complexes do not bind to Bax or PON1 DRE-like DNA elements in vitro. Guinea pig hepatic cytosol (A) or in vitro synthesized mouse AhR and ARNT (B) were incubated with DMSO (2% (v/v)), TCDD (20 nM), DMBA-DHD (1μM), quercetin (50 μM) or 3MC (5 μM) for 2-3 hours at 20°C followed by incubation with [32P]-labeled oligonucleotide probes. Protein-DNA complexes were resolved by EMSA and visualized by PhosphorImager and ImageQuant software analysis. The arrow indicates the position of the inducible AhR:ARNT:DNA complex.