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. Author manuscript; available in PMC: 2015 Jan 1.
Published in final edited form as: Arch Biochem Biophys. 2013 Nov 4;541:10.1016/j.abb.2013.10.021. doi: 10.1016/j.abb.2013.10.021

Figure 3.

Figure 3

Nuclear extracts from TCDD or DMBA-DHD treated mouse hepatoma cells do not exhibit ligand-inducible protein-DNA complexes with the Bax or mutant Bax DRE-like DNA element. Nuclear extracts were prepared from mouse hepatoma (hepa1c1c7) cells incubated with DMSO (0.1% (v/v)), TCDD (1 nM) or DMBA-DHD (1 μM) for 1 hour as described in Materials and Methods. Extracts (3 μg of protein) were incubated with the indicated [32P]-labeled DNA oligonucleotide probes and subjected to EMSA analysis to resolve the protein:DNA complexes. The arrow indicates the position of the induced AhR:ARNT:DNA complex.