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. Author manuscript; available in PMC: 2015 Jan 1.
Published in final edited form as: Arch Biochem Biophys. 2013 Nov 4;541:10.1016/j.abb.2013.10.021. doi: 10.1016/j.abb.2013.10.021

Figure 4.

Figure 4

Nuclear extracts from quercetin and 3MC exposed human HuH7 cells do not exhibit ligand-inducible protein-DNA complexes with the PON1 DRE-like element. Nuclear extracts were prepared from human hepatoma (HuH7) cells incubated with DMSO (0.1%), TCDD (10 nM), 3MC (5 μM) or quercetin (50 μM) for 1 hour. The extracts (3 μg of protein) were then incubated with [32P]-labeled DRE3 or PON1 oligonucleotide probes and subjected to EMSA to resolve the protein:DNA complexes. The arrow indicates the position of the induced protein:DNA complex.