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. Author manuscript; available in PMC: 2015 Jan 1.
Published in final edited form as: Arch Biochem Biophys. 2013 Nov 4;541:10.1016/j.abb.2013.10.021. doi: 10.1016/j.abb.2013.10.021

Figure 7. TCDD, 3MC and quercetin fail to stimulate luciferase reporter gene expression from the PON1 DRE-like DNA element.

Figure 7

Human hepatoma (HuH7) cells were transiently transfected with pGudLuc7.0 containing a single DRE3 or PON1 DNA oligonucleotide present upstream of the luciferase reporter gene or with a plasmid containing either 1000 base pairs of the upstream regulatory region from the PON1 gene (pPON1000-FL) or three tandem copies of the wild type PON1 DRE-like sequence (p(XRE-PON)3-FL) upstream of the luciferase reporter gene [13]. Cells were incubated with DMSO (0.1% (v/v)), TCDD (10 nM), 3MC (5 μM) or quercetin (50 μM) for 4 hours followed by measurement of luciferase activity. Values represent the mean ± SD of triplicate determinations and are representative of three independent experiments. The asterisk indicates a significant (p < 0.05; Student's t-test) increase in luciferase activity compared to the DMSO control.