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. Author manuscript; available in PMC: 2014 Oct 8.
Published in final edited form as: Phys Biol. 2013 Oct 8;10(5):10.1088/1478-3975/10/5/056010. doi: 10.1088/1478-3975/10/5/056010

Figure 1.

Figure 1

(A) NB4, HL60, AML14 and HEL cells were lysed and TF antigen levels detected with an ELISA. (B) Cells were incubated with FVIIa (10 nM) and the activation of FX (150 nM) was measured by tracking 405 nm light absorbance in the presence of Spectrozyme Xa®. (C) Cells were incubated with FXa (15 nM) and FVa (10 nM) and the activation of FII (200 nM) measured by tracking 405 nm light absorbance in the presence of Spectrozyme TH®. (D) AML cells were spiked into whole blood, and the time to occlusion measured in an ex vivo occlusive thrombus formation assay. Data are mean ± SE (n = 3). * p<0.05 versus buffer.