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. 2004 Apr;42(4):1534–1541. doi: 10.1128/JCM.42.4.1534-1541.2004

FIG. 1.

FIG. 1.

Amplification by real-time NASBA and differential beacon detection of two Ethiopian virus isolates. (a) Isolate F-0008 M grouped with genotype C by sequencing. (b) Isolate F-0337P grouped with genotype C′. The primers used for the amplifications were identical for both isolates, and a mixture of two different detection beacons was included in both reactions. The beacon specific to the C genotype was labeled with the fluorophore FAM and the beacon specific to the C′ genotype was labeled with the fluorophore ROX. Panel A shows the preferential fluorescence increase of the genotype C beacon, while panel B shows the preferential fluorescence increase of the genotype C′ beacon.