Table 1. Description of the culture medium composition to induce the tenogenic potential of hAFSCs and hASCs.
Medium | Description |
A) | Basic medium: α-MEM, FBS (10%), A/A (1%) |
B) | AFSCs expansion medium (BAFC): α-MEM +15% ES-FBS, 1% glutamine, 1% antibiotic, 18% Chang B and 2% Chang C |
C) | hASCs: basic medium A+glutamine (2 mM) and ascorbic acid (0.2 mM) |
D) | Basic medium A+glutamine (2 mM)+ascorbic acid (0.2 mM)+EGF (10 ng/ml) |
E) | Basic medium A+glutamine (2 mM)+ascorbic acid (0.2 mM)+bFGF (10 ng/ml) |
F) | Basic medium A+glutamine (2 mM)+ascorbic acid (0.2 mM)+PDGF-BB (10 ng/ml) |
G) | Basic medium A+glutamine (2 mM)+ascorbic acid (0.2 mM)+TGF-β1 (10 ng/ml) |
α-MEM : Minimum Essential Medium Eagle - Alpha Modification; FBS : fetal bovine serum; A/A : antibiotic/antimicotic solution; FBS-ES : fetal bovine serum embryonic screened; EGF : endothelial growth factor (Peprotech/100-15); bFGF : basic-fibroblast growth factor (Peprotech/100-18B); PDGF-BB : platelet-derived growth factor (eBioscience/14-8501); TGF-β1 : transforming growth factor-β1 (eBioscience/14-8348).