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. 2013 Dec 30;8(12):e83713. doi: 10.1371/journal.pone.0083713

Figure 6. Effect of signaling pathway inhibitors on ox-LDL-induced Klotho downregulation in proximal tubular cells.

Figure 6

Pretreatment of MCT with parthenolide (1 µM) or PD098059 (50 µM) for 1 hour attenuates the decrease in Klotho mRNA (A) and protein (B) induced by ox-LDL (25 µg/mL). (C) Treatment with ox-LDL (25 µg/mL) promotes IκB degradation (left panel) and ERK1/2 phosphorylation (right panel) in MCT cells. Cell lysates were analyzed by Western blot for IκB and phospho-ERK 1/2. Each blot was stripped and reprobed with beta-actin and anti-ERK antibody, respectively. Mean±SD of 3 independent experiments. *p<0.05 vs control, # p<0.05 vs ox-LDL.