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. 2013 Dec 23;9:183–195. doi: 10.2147/IJN.S49460

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© 2014 Kamath et al. This work is published by Dove Medical Press Limited, and licensed under Creative Commons Attribution – Non Commercial (unported, v3.0) License

The full terms of the License are available at http://creativecommons.org/licenses/by-nc/3.0/. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed.

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Immunophenotyping of cell surface markers of hBMSCs using flow cytometric analysis. (I) Positive markers for mesenchymal stem cells: (A) CD90-PERCP, (B) CD105-APC, (C) CD73-PE, (D) CD44-FITC, (E) CD29-PE, showing higher percentage expression (control and sample as labeled). (II) Negative markers for mesenchymal stem cells: (A) CD34-PE, (B) CD45-APC-Cy7, (C) CD133-APC-A, (D) CD31-FITC, (E) HLA-DR-PERCP show a low percentage expression (control and sample as labeled).

Note: The surface marker profile shows that the cells obtained at third passage were mesenchymal stem cells. BM1-P3-DMEM-LG represents bone marrow cells obtained at third passage in DMEM-LG medium.

Abbreviations: DMEM-LG, Dulbecco’s Minimum Essential Medium-Low Glucose; hBMSCs, human bone marrow-derived mesenchymal stem cells; SSC, side scatter; BM, bone marrow.

Immunophenotyping of cell surface markers of hBMSCs using flow cytometric analysis. (I) Positive markers for mesenchymal stem cells: (A) CD90-PERCP, (B) CD105-APC, (C) CD73-PE, (D) CD44-FITC, (E) CD29-PE, showing higher percentage expression (control and sample as labeled). (II) Negative markers for mesenchymal stem cells: (A) CD34-PE, (B) CD45-APC-Cy7, (C) CD133-APC-A, (D) CD31-FITC, (E) HLA-DR-PERCP show a low percentage expression (control and sample as labeled).

Note: The surface marker profile shows that the cells obtained at third passage were mesenchymal stem cells. BM1-P3-DMEM-LG represents bone marrow cells obtained at third passage in DMEM-LG medium.

Abbreviations: DMEM-LG, Dulbecco’s Minimum Essential Medium-Low Glucose; hBMSCs, human bone marrow-derived mesenchymal stem cells; SSC, side scatter; BM, bone marrow.