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Characterization of hBMSCs at third passage. (A) Morphology of cells on day 7 of culture. (B) Morphology of hBMSCs on day 14, showing 80% confluency. (C) von Kossa staining for calcium deposits shown as black-colored crystals after osteogenic differentiation and (D) Oil O Red stains of lipids after adipogenic differentiation.
Note: All images were obtained under light microscopy at 100× magnification.
Abbreviation: hBMSCs, human bone marrow-derived mesenchymal stem cells.
