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. 2013 Dec 30;8(12):e84137. doi: 10.1371/journal.pone.0084137

Figure 1. Observation and verification of N. bombycis oral-infected silkworms.

Figure 1

(A) Survival curves and body length profiles of N. bombycis oral-infected silkworms. Negative: Uninfected silkworms reared under 25°C at 70% humidity. Exp 1,Exp2 and Exp3: silkworms fed with 5×104, 1×105 and 3×105 spores per larvae, respectively. (B) Observation of spores in the midgut at different stages using optical microscopy. Cont: The midgut of an uninfected silkworm. Exp: Midgut from an infected silkworm. dpi: days post-infection. (C) Validation of infection by RT-PCR analysis. Midgut samples were randomly selected from the dissected larvae of N.bombycis infected set or the control set. The total RNAs for the analysis of microarray were also used to generate the sscDNA for RT-PCR to verify the infection. Exp: Infected larvae. Cont: Silkworm without infection. β-tubulin: A constitutively expressed protein coding gene (β-tubulin) of N. bombycis, Genbank ID: DQ663475.1. sw22934: A conserved silkworm housekeeping gene (BmTIF2), Genbank ID: NM_001043911.