Figure 2. Characteristics of adipocytes isolated from different fat depots of the control and cold-acclimated mice.

Adipocytes were isolated from interscapular brown adipose tissue (BA), inguinal (I-WA) and perigonadal white adipose tissue (G-WA) of the control and cold-acclimated mice. (A) Typical photomicrographs of adipocytes isolated form each depot of the control or cold-acclimated mice. Arrows indicate multilocular beige adipocytes. (B) Expression of several genes in the isolated adipocytes was measured by quantitative real-time PCR. Expression levels normalized to ß-actin expression are shown. White and black columns indicate the control and cold-acclimated groups, respectively. Values are means ± SE for 9 mice. Different letters indicate significant differences between the groups (p<0.05).