porB PCR-AREA patterns observed following gel electrophoresis of AluI and SspI double digests of the porB PCR product. The PCR product was amplified from meningococci with the porB VR1-4 and VR2-D sequences (lanes 2, 3, 7, 9, 12, and 13), the porB VR1-19 and VR2-D sequences (lanes 4 and 11), the porB VR1-19 and VR2-A sequences (lanes 8, 10, and 14), and the porB VR1-4 and VR2-B sequences (lanes 5 and 6). Molecular size markers (100 bp; Roche) are shown in lane 1.