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. 2013 Dec 30;8(12):e85199. doi: 10.1371/journal.pone.0085199

Figure 2. Landmarks (LMs) placement (A) and thin-plate-spline transformations (B) for Ligia hawaiensis (clades D, E, F).

Figure 2

A. LMs 1 and 25 represent the posterior margin of the eyes at the body perimeter; LMs 2-11 and 15–24 are the posterior-most point of each segment; LMs 12 and 14 are the lateral posterior points of the pleotelson, whereas LM 13 is the posterior-most point of the pleotelson; LMs 26 and 27 correspond to the inner-most margin of the eyes. B. Thin-plate-spline transformations of LM positions are shown magnified (10 ×) to reach the natural extremes observed in the clades, which are also indicated with images for specimens with the highest probability of being correctly assigned to each clade.