Figure 7. Western blots of whole protein extracts from RWPE-1, LNCaP, PC3 and DU145 cell lines.

The prostate cell lines were cultivated in regular medium until 100% confluence and individually processed for protein extraction. After electrophoresis, the proteins were transferred onto a nitrocellulose membrane and probed with an anti-androgen receptor (AR) antibody. After incubation with the secondary antibody, the bands were visualized with DAB. All cell lines employed in this investigation expressed AR except for the DU145 cell line. Experiments were carried out in triplicate. (MW) Molecular Weight Standard. The estimated MW of AR is approximately 110 kDa.