Skip to main content
. 2013 Dec 30;8(12):e84775. doi: 10.1371/journal.pone.0084775

Figure 3. SigX levels are controlled at protein and mRNA levels in the overexpression strain ALB04.

Figure 3

A. Immunobloting using a polyclonal anti-SigX antibody and total protein extracts obtained from cultures growing in the presence of arabinose and gentamycin. The Spectra Multicolor Broad Range Protein Ladder (Fermentas) was used as molecular weight marker (MWM). B and C. Quantitative RT-PCR using cDNA made by reverse transcription of total RNA from ALB01 (control), ALB04 (sigX overexpression) or ALB02 (PA14_21550 overexpression) cells growing in inducing conditions and specific sigX (B) or PA14_21550 (C) primers. Asterisks indicate significant differences comparing to the reference sample ALB01 6h, plotted as 1 (p<0.01).