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. 2013 Dec 30;8(12):e84775. doi: 10.1371/journal.pone.0084775

Figure 5. Lower levels of overexpression and natural induction of sigX induce target genes.

Figure 5

A. Arabinose was added to ALB04 cultures at exponential phase and samples (+Ara) were collected after 15, 30 and 180 minutes for RNA extraction and qRT-PCR of sigX, accA and fabH3. Control samples were taken at the same time points (-Ara). All values are shown as relative to t=0 for each treatment, set as 1. B. β-galactosidase activity assay of sigX and fabH3-lacZ promoter fusions in the wild-type background (PA14::sigX_lacZ and PA14::fabH3_lacZ) in LB with 171 mM NaCl or without NaCl. The values plotted are averages of biological triplicates and error bars represent standard deviations.