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. 2013 Dec 30;8(12):e84775. doi: 10.1371/journal.pone.0084775

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© 2013 Boechat et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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A. Genomic region containing fabH3 and a sketch showing the PfabH3-lacZ fusion. B. qRT-PCR of PA14_21550 in sigX overexpression (ALB04) and control (ALB01) strains. C. β-galactosidase activity assay of fabH3 promoter-lacZ fusion in strains overexpressing PA14_21550 (ALB02::fabH3_lacZ) and sigX (ALB04::fabH3_lacZ). The values plotted are averages of biological triplicates and error bars represent standard deviations. D. qRT-PCR of some sigX overexpression targets in PA14_21550 overexpression strain (ALB02). The values shown in B and D are averages of technical triplicates of at least two independent assays and error bars represent standard deviations.