Table 1. Fatty acid biosynthesis and other selected proteins differentially expressed in ALB04.
| Protein | Function a | ALB04 3h/ ALB01 3h b | ALB04 28h/ ALB01 3h c |
|---|---|---|---|
| Fatty acid biosynthesis | |||
| PA14_01240 | carbonic anhydrase | 5.77 | ND |
| PA14_17270 (AccA) | acetyl-CoA carboxylase carboxyltransferase subunit alpha | 1.98 | ND |
| PA14_64100 (AccB) | acetyl-CoA carboxylase biotin carboxyl carrier protein subunit | 4.17 | ND |
| PA14_23860 (AccD) | acetyl-CoA carboxylase subunit beta | 2.12 | ND |
| PA14_25650 (FabD) | malonyl-CoA-[acyl-carrier-protein] transacylase | 6.42 | 2.40 |
| PA14_68360 (FabY) | β-oxoacyl synthase I/II | 4.19 | 0.28 |
| PA14_25660 (FabG) | β-oxoacyl-(acyl-carrier-protein) reductase | 3.30 | 0.63 |
| PA14_57050 (FabG2) | β-oxoacyl-(acyl-carrier-protein) reductase | 6.80 | 0.78 |
| PA14_25900 (FabV) | trans-2-enoyl-CoA reductase | 5.24 | 0.76 |
| PA14_21540 (FabH3) | β-oxoacyl-(acyl carrier protein) synthase III | 4.12 | 1.19 |
| PA14_43690 (FabB) | β-oxoacyl -(acyl carrier protein) synthase I | 1.69 | 1.95 |
| PA14_46490 (FabF2) | β-oxoacyl-(acyl carrier protein) synthase II | 5.12 | 1.86 |
| Other | |||
| PA14_41575 (SigX) | RNA polymerase sigma factor SigX | 22.72 | 1.88 |
| PA14_17310 (KdsA) | 2-dehydro-3-deoxyphosphooctonate aldolase (LPS synthesis) | 6.14 | 2.08 |
| PA14_05460 (BioA) | adenosylmethionine-8-amino-7-oxononanoate transaminase (cofactor synthesis) | 4.19 | ND |
| PA14_12130 (Lis) | lipoyl synthase (cofactor synthesis) | 2.09 | 0.42 |
| PA14_63250 (YcfY) | acetyl-CoA acetyltransferase (FA degradation) | 1.18 | 3.46 |
| PA14_62130 (IlvC) | ketol-acid reductoisomerase (CoA synthesis) | 1.11 | 1.44 |
| PA14_41570 (OprF) | major porin and structural outer membrane porin OprF | 0.77 | 4.40 |
b fold change in protein amounts in SigX overexpressing cells after 3h of arabinose induction in comparison to the control strain.
c fold change in protein amounts in recovered cells (ALB04 after 28h of arabinose induction)in comparison to control cells (ALB01) after 3h of arabinose induction.