Figure 7. Luciferase assay monitoring of the KCNE1 / KCNB2 - miR-1 interaction.

HEK293 cells were co-transfected with miR-1- or AMO-1-plasmids and KCNE1 or KCNB2- plasmids and the mutation studies by replacing the matching site in miR-1 and the 3’UTRs of KCNE1/KCNB2 were performed as described in the Materials and Methods section. Luciferase activity, measured in the samples indicated on the “X” axis, was normalized to the activity exhibited in the control cells. * P < 0.05 vs. Ctl, † P < 0.05 vs. miR-1, one-way ANOVA and the Tukey’s post-hoc test.