Table 2. The primers for qRT-PCR.
Gene | Accession no. | Sequence |
---|---|---|
miR-1 | NSCUT00000020310 | F:5'--CTCAACTGGTGTCGTGGAGTCGGCAATTCAGTTGAGATACACAC--3' |
R:5'--ACACTCCAGCTGGGTGGAATGTAAAGAAGT--3' | ||
β-actin | NM_001101683.1 | F:5'- -ACCACAGTCCATGCCATCAC--3' |
R: 5'--TCCACCACCCTGTTGCTGTA--3' | ||
KCNB2 | NM_001082137.1 | F: 5'--CCTGGCGCTGCGGCTTTGTCC--3' |
R: 5'-- AGGCGTGTCCGGGGCAGTCG--3' | ||
KCNE1 | NM_001109822.1 | F: 5'--AACCGCCCGGACCCCAACAC--3' |
R: 5'--TTCCCGGACACGATGCCACAGG--3' |
F, forward primer; R, reverse primer