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. 2013 Dec 30;8(12):e85589. doi: 10.1371/journal.pone.0085589

Figure 3. Nrp1+ T cells are not proliferating and have no regulatory activity.

Figure 3

(A) Ki67 expression in tonsillar CD3+ CD4+ Nrp1- and Nrp1+ T cells. Five tonsils were analyzed and the expression data was compared with a Student’s paired t-test (**: p≤0.01). (B) The regulatory function of Nrp1+ Tfh cells was tested in coculture experiments with CFSE-labeled non-Tfh cells as described in Materials and Methods. The percentage of non-Tfh cells having diluted CFSE after 5 days of culture with various ratios of Nrp1+ Tfh cells was analyzed by flow cytometry. Data pooled from three distinct experiments are summarized here.