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. 2013 Dec 30;8(12):e85589. doi: 10.1371/journal.pone.0085589

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© 2013 Renand et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Cell suspensions from malignant lymph node biopsies of 5 AITL patients (P1-P5) and one non-malignant reactive lymph node (LN 1) were analyzed for CXCR5 and PD-1 expression (A), and for Nrp1 and PD-1 expression (B) in gated CD3⁺ CD4⁺ T cells. (C) Bar graph display of percentage of Nrp1⁺ cells among PD1⁺ CD4⁺ T cells, serum gammaglobulin concentration and medullar plasmacytosis (percentage plasma cells among bone marrow cells) for each patient. Note that only P3 shows a significant proportion of Nrp1⁺ malignant T cells, high gammaglobulinaemia, and important medullar plasmacytosis.