FIG. 1.

Southern blot analysis of representative isolates from the three groups. Genomic DNA (5 μg) of L. donovani from groups K, P, and M was digested for 16 h at 37°C with MvaI (4 U/μg of DNA; MBI Fermentas) and resolved on a 1% agarose gel, and blots were probed with ³²P-labeled LdP13 and visualized by autoradiography. The numbers below the lanes indicate the numbers of isolates falling in the respective categories.