Figure 2. IL-4 enhances paracellular permeability in a Jak-dependent manner. 16HBE cells were grown to confluence and then incubated with IL-4 (50 ng/ml for 72 h), followed by analysis of paracellular flux to 3-kDa-FITC-dextran added to the apical chamber. Cells were also incubated with wortmannin or different JAK inhibitors as indicated. Data are the mean ± SEM of n = 3−4 experiments. *p < 0.05 compared with control cells. #p < 0.05 compared with cells treated with IL-4 alone.