Figure 3. IL-4 disrupts apical junctional complex structures. 16HBE cells were incubated with IL-4 (50 ng/ml for 72 h) followed by analysis of AJC structure using immunofluorescence and antibodies directed against ZO-1, Occludin, E-Cadherin, β-Catenin or Claudin- 4. Results are from one experiment representative of n = 2. Scale bar = 30 microns.