Figure 4. IL-4 does not induce claudin- 2 expression or major changes in AJC protein expression. 16HBE cells were incubated without (-) or with (+) IL-4 (50 ng/ml) for 72 h, and then whole cell lysates were analyzed by western blot for expression of the indicated junctional proteins. GAPDH was used as lane loading control. The asterisk in the Claudin-2 immunoblot represents lysates from Caco-2 cells used as a positive control. Results are from one experiment in representative of n = 2−3.