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. Author manuscript; available in PMC: 2014 Sep 26.
Published in final edited form as: Adv Mater. 2013 Mar 26;25(21):2897–2902. doi: 10.1002/adma.201205237

Figure 2.

Figure 2

Optimization and validation of the PN-NanoVelcro Chips using CellSearch™ Calibration sample (pre-stained SK-BR-3 breast cancer cells) and cell lines. a) The cell-capture efficiency of PN-NanoVelcro Chip with different electrospining time was assessed. CellSearch™ calibration samples containing 100 pre-stained EpCAM-positive SK-BR-3 cells were spiked into PBS as a model system. b) Cell-capture efficiency of PN-NanoVelcro Chip at flow rates of 0.5, 1, 2, 3, 4 mL h-1. c) The distributions of captured cells on the PN-NanoVelcro Chips were assessed in PBS and normal blood. d) Comparison of the capture performance between PN-NanoVelcro Chip and four different controls: i) PN-NanoVelcro Chip without anti-EpCAM; ii) PN-NanoVelcro Chip without chaotic mixing (flat channel without herringbone structure); iii) Anti-EpCAM coated PLGA thin film with electrospining; iv) SiNW NanoVelcro Chip coated with anti-EpCAM. e) Capture efficiencies at different spiking cell numbers ranging from 10-100 mL-1. f) Capture efficacies of three different PC cell lines, PC3, C4-2 and LNCaP in PBS and normal blood. All error bars show standard deviations (n>=3).