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. Author manuscript; available in PMC: 2014 Sep 24.
Published in final edited form as: ACS Nano. 2013 Aug 12;7(9):7711–7723. doi: 10.1021/nn402241b

Figure 1.

Figure 1

Chronic exposure to SWCNT, MWCNT and asbestos induces cell proliferation and aggressive behavior of mesothelial MeT5A cells. (A) Passage control (Control), vehicle control (Survanta) and chronic SWCNT, MWCNT or asbestos-exposed cells were seeded in 96 well plate and cell proliferation assay was performed using Cyquant® cell proliferation kit for 3 continuous days. (B) Hoechst 33342 vital stain of passage control, vehicle control and chronic SWCNT, MWCNT or asbestos-exposed cells at 1 and 3 days after seeding. (C) Cell invasion and migration of passage control, vehicle control and chronic SWCNT, MWCNT or asbestos-exposed cells were determined using Transwell® with 8 μm pore size polycarbonate filter and BD Matrigel® invasion chamber. Cells that invaded or migrated to the lower side of the membrane were stained. (D) The invading and migrating cell numbers were then quantified by counting and depected as bar charts. * = significant difference from control with P<0.05, # = significant difference from asbestos-exposed cells with P<0.05