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. Author manuscript; available in PMC: 2014 Sep 24.
Published in final edited form as: ACS Nano. 2013 Aug 12;7(9):7711–7723. doi: 10.1021/nn402241b

Figure 3.

Figure 3

Increased MMP-2 expression and activity in chronic CNT-exposed and asbestos-exposed MeT5A cells. (A) MMP-2 expression in passage control, vehicle control and chronic SWCNT, MWCNT or asbestos-exposed cells was determined by Western blotting. Lysates of each sample were resolved under denaturing conditions by 10% SDS-PAGE and transferred onto PVDF membrane. The membrane was then probed with MMP-2 antibody and detected by enhanced chemiluminescence detection system. * = significant difference with P<0.05 (B) MMP-2 gelatinolytic activity of passage control, vehicle control and chronic SWCNT, MWCNT or asbestos-exposed cells was shown by gelatin zymography. Cell supernatants were collected, resolved by electrophoresis in SDS-polyacrylamide gels containing 0.1% gelatin and stained with 0.5% Coomassie brilliant blue. Densitometry was used to compare enzymatic activity. * = significant difference with P<0.05 (C) Cellular MMP-2 expression was visualized by immunofluorescence staining. Cells were cultured on glass cover slips, fixed in 4% paraformaldehyde and stained for F-actin (green) and MMP-2 (red). MMP-2 expression level represented by red fluorescence intensity per square inches of each sample was indicated at lower right corner of each capture.