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. Author manuscript; available in PMC: 2015 Mar 1.
Published in final edited form as: Amino Acids. 2013 Aug 1;46(3):565–573. doi: 10.1007/s00726-013-1565-2

Fig. 7.

Fig. 7

A: Serum starved IEC-6 cells were incubated in DMEM in the absence of amino acids (AA-) in the absence and presence of ASN for the times indicated. Cell extracts were analyzed by Western blot to determine the levels of phosphoserine 473-AKT, AKT, AZ and actin. B: Cells were incubated with the indicated concentrations of PP242 for 4h, and extracts were analyzed for levels of phosphoserine 473-AKT, phosphoserine 235/236-S6 ribosomal protein, AZ and actin. Representative Western blots from three observations. From: Ray et al 2012.