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. 2013 Apr 10;1(3):e24517. doi: 10.4161/tisb.24517

Table 3. Oligonucleotide primers used for RT-PCR.

Gene Gene ID Primer Sequence Product Size
cClaudin-2 GI:118089520 Forward 5′-ccatggtctctatgggactcc-3′ 684
    Reverse 5′-gcttctacacgtatcccgtc-3′  
cClaudin-3 GI:46049088 Forward 5′-gcccatgtggagggtgac-3′ 376
    Reverse 5′-caccagcgggttgtagaaat-3′  
cClaudin-4 GI: 363741048 Forward 5′-cgggatccgatggcctccatggggct-3′ 622
    Reverse 5′-gtggaattccttacacgtagttgctg-3′  
cClaudin-8 GI:50730032 Forward 5′-atgatcagtggtgcgtgccaaattgc-3′ 675
    Reverse 5′-ctagacgtactgactcttagagtagg-3′  
cClaudin-10 GI:118084678 Forward 5′-catggcgagcacgtcggcggag-3′ 687
    Reverse 5′-ttaaacgtaagcgttcttgtc-3′  
cClaudin-11 GI:118095299 Forward 5′-catgggtggccacctgcctgca-3′ 608
    Reverse 5′-ttcagacgtgggcgctctta-3′  
cClaudin-12 GI:118085731 Forward 5′-atgggctgcagggatgttcat-3′ 738
    Reverse 5′-cttaagatgtgtgtgtcacaaca-3′  
cClaudin-14 GI:118083818 Forward 5′-atggcaagcacggccgttcagttac-3′ 708
    Reverse 5′-tcacacatagtcattcaatctgtagc-3′  
cClaudin-15 GI:50751942 Forward 5′-atggcttcatcttctctacag-3′ 675
    Reverse 5′-ttagatgtactccttg-3′  
cClaudin-16 GI:50752418 Forward 5′-atgccactggatgtaccaacacc-3′ 744
    Reverse 5′-tcacactcttgtatccacggc-3′  
cClaudin-17 GI:118083832 Forward 5′-catggcttgctgtgcattac-3′ 669
    Reverse 5′-gccttctatacgtatgcctgc-3′  
cClaudin-18 GI:118094873 Forward 5′-atgtccacgaccctctggc-3′ 780
    Reverse 5′-ctagacgtactgactcttagagtagg-3′  
cClaudin-20 GI:118088400 Forward 5′-ccgaactggaaggtaaatgc-3′ 522
    Reverse 5′-catgctccttgctgctttttg-3′  
cClaudin-22 GI:118101949 Forward 5′-atgctactggcgctcttcgg-3′ 654
    Reverse 5′-cagattaccaggtcagcg-3′  
cClaudin-23 GI: 50746852 Forward 5′-atgcggacaccggccgtgat-3′ 702
    Reverse 5′-tcatcttggggtctgg-3′