Figure 3. The effect of PD0332991 on proliferation and replicative potential (RP) in MEL10 cells. Cells were treated with the indicated concentrations of PD0332991 with or without 10 nM rapamycin. (A and B) After 3.5 days, proliferation (A) was measured by cell counting, as fold increase compared with initial (seeding) cell numbers. In parallel sets (B), drugs were washed out, and cells were allowed to proliferate, and RP was measured after 7 days of culture, as described in “Materials and Methods”. (C and D) After 7 days treatment, proliferation (A) was measured by cell counting, as fold increase compared with initial (seeding) cell numbers. In parallel sets (B), drugs were washed out, and cells were allowed to proliferate, and RP was measured after 7 days of culture, as described in “Materials and Methods”.