Figure 5.

The effect of PD0332991 on RPE cells. (A). Immunoblot analysis: Cells were treated with the indicated concentrations of PD0332991 with or without 10 µM U0126. Cells were lysed after 24 h. (B and C) Proliferation (B) and RP: replicative potential (C). After 7 days treatment proliferation (B) was measured by cell counting. In parallel set (C), drugs were washed out and cells were allowed to proliferate. RP was measured by counting after 6 days of culture. (D) The effect of rapamycin on RP of PD0332991-treated RPE cells. Cells were treated with 0.2 or 2 µM PD0332991 with or without 5 nM rapamycin (R). After 5 days treatment drugs were washed out and RP was determined.