Figure 1. Transient HA-tagged TPD52 transfection of SK-BR-3 cells disrupted DNA damage repair post-IR. (A) SYBR Green-stained DNA in un-irradiated cells (no IR), cells harvested immediately (0 h) or 16 h post 2 Gy IR (16 h). Composite images shown are from one of 3 independent experiments. (B) DNA damage was quantified by comparing mean ± SE olive tail moments (y-axis, arbitrary units) at different time points. Experiments were performed in triplicate and images from 3 independent experiments (47–54 comet specimens per condition per experiment) were quantified. *P = 0.016, Student t test. (C) Representative western blots indicate endogenous TPD52 and HA-TPD52 levels in SK-BR-3, vector-, or HA-TPD52-transfected cells. GAPDH served as a loading control. MW, molecular weight in kDa (right).