Figure 7. Interactions between TPD52 and ATM. (A) GST pull-down assays using GST-tagged full-length mouse Tpd52 or human TPD52 or GST tag, and MCF-7 (left) or SK-BR-3 (right) cell lysates. Upper and middle panels show the results of western blot analyses using ATM antibody and TPD52L1 antisera, respectively. The lower panel shows Ponceau S staining to reveal GST-Tpd52/TPD52 (arrowheads) or GST tag (arrow). At least 3 independent experiments were performed. (B) Schematic representations of deleted Tpd52 recombinant proteins. Amino acid coordinates for each motif/domain are indicated. (C) Pull-down assays using thioredoxin-6His-tagged Tpd52-deleted proteins and SK-BR-3 cell lysates. Upper and middle panels show results of western blot analyses using ATM antibody and TPD52L1 antisera, respectively. The lower panel shows Ponceau S staining to reveal His-Tpd52 proteins or His-thioredoxin tag (arrow). Results shown are representative of those obtained from 3 independent experiments. (D) Sequence of TPD52 aa 109–134 with broken lines indicating FHA and BRCT motifs as predicted through ELM. S¹¹¹ and S¹³¹ are underlined.