Figure 9. MG132 treatment did not increase Atm levels in 3T3 cell lines. Vector and TPD52-expressing (D52-1-12) cell lines were treated with 50 μM MG132 (4 h, 6 h, 8 h) or DMSO vehicle (0 h) and harvested at the indicated time points. Total proteins were subjected to western blot analyses with the indicated antisera (left). Results shown are representative of 3 independent experiments.