Figure 2.

The Long-Period Phenotype in hos1 Does Not Require Selected Individual Clock Components.

(A) Delayed fluorescence assays comparing the wild type (Col, black circles) to hos1 (gray circles) and the single (white circles) and double (red circles) mutants. Seedlings were entrained for 16 d in 12L:12D white-light cycles at 22°C before transfer to experimental conditions of constant 12, 17, or 27°C and constant red light. Plots show period estimations produced via mathematical analysis plotted versus RAE. Data are from individual wells containing 10 to 20 seedlings.

(B) Plots illustrating the effect of temperature on the variable weighted mean of the period for the wild type (Col, black circles) to hos1 (gray circles) and the single (white circles) and double (red circles) mutants. Data are variable weighted mean ± se. Stars indicate significant difference to the respective wild type as measured by Student’s t test where one star indicates P ≤ 0.05 and two stars P ≤ 0.01.